Journal: PLoS ONE

Article Title: Collagenase mRNA Overexpression and Decreased Extracellular Matrix Components Are Early Events in the Pathogenesis of Emphysema

doi: 10.1371/journal.pone.0129590

Figure Lengend Snippet: Antibodies used in immunohistochemistry studies.

Article Snippet: The following primary antibodies were used in this study: a rabbit polyclonal anti-mouse fibrillin 1 (ab21618) (1:200, Abcam, Cambridge, MA, EUA) and an anti-mouse elastin A-19 (SC-17580) (1:600, Santa Cruz Biotechnology, Santa Cruz, CA, USA).

Techniques: Immunohistochemistry, Plasmid Preparation

Journal: International Journal of Medical Sciences

Article Title: Transcriptome Profile of Human Fibroblasts in an Ex Vivo Culture

doi: 10.7150/ijms.35693

Figure Lengend Snippet: a) Autologous fibroblasts in the second passage. Cell division in a monolayer of fibroblasts (A); thin elastic fibers between cells (B); and collagen type I with a red-yellowish color and strong birefringence (C). Safranin O/Fast green; B. Weigert's method; C. Sirius Red. Objective magnification: x 40. b) Morphology of skin. Arrangement of type I collagen bundles in the papillary dermis and the reticular dermis (A) stained with H-E; localization of type III collagen (reticular fibers) after silver staining (B) and thin collagen bundles in the dermis with predominantly yellowish color (C). (A x 20; B x10, C x 10). c) Immunolocalization of fibrillin-1-rich microfibrils (yellow arrows) (A, B) and cytochrome P450 aromatase in fibroblasts -yellow arrows (C). (A x 20; B x 40. C x 40).

Article Snippet: After slow cooling to room temperature, the slides were washed twice in PBS for 5 min and then incubated for 60 min with primary mouse anti-human fibrillin-1 antibody (AbD Serotec MorphoSys AbD GmbH, Germany).

Techniques: Staining, Silver Staining